Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- HYB 211-01-02 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NGAL Monoclonal Antibody (5G5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- HYB 211-01-02 is specific for human NGAL, native and denatured forms. No cross-reaction to NGAL from other species has been detected. HYB 211-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. HYB 211-01-02 reacts strongly with NGAL. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against the antigen. In immunohistochemical staining of paraformaldehyde fixed neutrophils HYB 211-01-02 recognizes NGAL. In western blotting HYB 211-01-02 reacts with NGAL in both reduced as well as unreduced form. The HYB 211-01-02 immunogen is recombinant human NGAL in monomer and dimer forms absorbed to aluminium hydroxide gel. The epitope specificity of HYB 211-01-02 differs from that of HYB 211-05-02, and differs from HYB 211-02-02 but overlaps.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G5
- Vial size
- 400 µL
- Concentration
- 0.94 mg/mL
- Storage
- 4° C, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA of NGAL using HYB 211-01 as the capture antibody and HYB 211-02B as the biotinylated detection antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NGAL in normal pancreas tissue using a NGAL monoclonal antibody (Product # HYB 211-01-02).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NGAL in chronic pancreatitis tissue using a NGAL monoclonal antibody (Product # HYB 211-01-02).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NGAL in tissue microarrays from human PaCa (MDACC) using a NGAL monoclonal antibody (Product # HYB 211-01-02).